Download or read book A Multi omics Perspective on Osmoadaptation and Osmoprotection in Bacillus subtilis written by Michael Kohlstedt and published by Cuvillier Verlag. This book was released on 2014-09-12 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt: In its natural environment, the soil bacterium Bacillus subtilis permanently encounters nutrient limitations and increases in osmotic stress. The present study investigated the systemswide response of B. subtilis to different simultaneously imposed stresses by combining chemostat experiments under conditions of carbon and nitrogen limitation, ionic and nonionic osmotic stress and osmoprotection with multi-omics analyses of the transcriptome, proteome, metabolome and fluxome. Surprisingly, the flux through central carbon and energy metabolism is very robust under all conditions studied. The key to achieve this robustness is the increased production of several enzymes of central carbon metabolism to compensate for their reduced activity in the presence of high salt. A major response of the cell during osmotic stress is the production of the compatible solute proline through the concerted adjustment of multiple reactions around the 2-oxoglutarate node, which drives metabolism towards the precursor glutamate. Co-regulations between the individual cellular components under the investigated stress conditions indicate that the fine-tuning of the transcriptional and metabolic networks involves functional modules that outreach single pathways. Additionally, the work shortly describes the salinity-decoupled fed-batch production of the compatible solute ectoine in Corynebacterium glutamicum and the contribution of precursor amino acids of the aspartate family.
Download or read book Stress and Environmental Regulation of Gene Expression and Adaptation in Bacteria written by Frans J. de Bruijn and published by John Wiley & Sons. This book was released on 2016-07-13 with total page 1472 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bacteria in various habitats are subject to continuously changing environmental conditions, such as nutrient deprivation, heat and cold stress, UV radiation, oxidative stress, dessication, acid stress, nitrosative stress, cell envelope stress, heavy metal exposure, osmotic stress, and others. In order to survive, they have to respond to these conditions by adapting their physiology through sometimes drastic changes in gene expression. In addition they may adapt by changing their morphology, forming biofilms, fruiting bodies or spores, filaments, Viable But Not Culturable (VBNC) cells or moving away from stress compounds via chemotaxis. Changes in gene expression constitute the main component of the bacterial response to stress and environmental changes, and involve a myriad of different mechanisms, including (alternative) sigma factors, bi- or tri-component regulatory systems, small non-coding RNA’s, chaperones, CHRIS-Cas systems, DNA repair, toxin-antitoxin systems, the stringent response, efflux pumps, alarmones, and modulation of the cell envelope or membranes, to name a few. Many regulatory elements are conserved in different bacteria; however there are endless variations on the theme and novel elements of gene regulation in bacteria inhabiting particular environments are constantly being discovered. Especially in (pathogenic) bacteria colonizing the human body a plethora of bacterial responses to innate stresses such as pH, reactive nitrogen and oxygen species and antibiotic stress are being described. An attempt is made to not only cover model systems but give a broad overview of the stress-responsive regulatory systems in a variety of bacteria, including medically important bacteria, where elucidation of certain aspects of these systems could lead to treatment strategies of the pathogens. Many of the regulatory systems being uncovered are specific, but there is also considerable “cross-talk” between different circuits. Stress and Environmental Regulation of Gene Expression and Adaptation in Bacteria is a comprehensive two-volume work bringing together both review and original research articles on key topics in stress and environmental control of gene expression in bacteria. Volume One contains key overview chapters, as well as content on one/two/three component regulatory systems and stress responses, sigma factors and stress responses, small non-coding RNAs and stress responses, toxin-antitoxin systems and stress responses, stringent response to stress, responses to UV irradiation, SOS and double stranded systems repair systems and stress, adaptation to both oxidative and osmotic stress, and desiccation tolerance and drought stress. Volume Two covers heat shock responses, chaperonins and stress, cold shock responses, adaptation to acid stress, nitrosative stress, and envelope stress, as well as iron homeostasis, metal resistance, quorum sensing, chemotaxis and biofilm formation, and viable but not culturable (VBNC) cells. Covering the full breadth of current stress and environmental control of gene expression studies and expanding it towards future advances in the field, these two volumes are a one-stop reference for (non) medical molecular geneticists interested in gene regulation under stress.
Download or read book The Perfect Slime written by Hans-Curt Flemming and published by IWA Publishing. This book was released on 2016-09-15 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Perfect Slime presents the latest state of knowledge and all aspects of the Extracellular Polymeric Substances, (EPS) matrix – from the ecological and health to the antifouling perspectives. The book brings together all the current material in order to expand our understanding of the functions, properties and characteristics of the matrix as well as the possibilities to strengthen or weaken it. The EPS matrix represents the immediate environment in which biofilm organisms live. From their point of view, this matrix has paramount advantages. It allows them to stay together for extended periods and form synergistic microconsortia, it retains extracellular enzymes and turns the matrix into an external digestion system and it is a universal recycling yard, it protects them against desiccation, it allows for intense communication and represents a huge genetic archive. They can remodel their matrix, break free and eventually, they can use it as a nutrient source. The EPS matrix can be considered as one of the emergent properties of biofilms and are a major reason for the success of this form of life. Nevertheless, they have been termed the “black matter of biofilms” for good reasons. First of all: the isolation methods define the results. In most cases, only water soluble EPS components are investigated; insoluble ones such as cellulose or amyloids are much less included. In particular in environmental biofilms with many species, it is difficult to impossible isolate, separate the various EPS molecules they are encased in and to define which species produced which EPS. The regulation and the factors which trigger or inhibit EPS production are still very poorly understood. Furthermore: bacteria are not the only microorganisms to produce EPS. Archaea, Fungi and algae can also form EPS. This book investigates the questions, What is their composition, function, dynamics and regulation? What do they all have in common?
Download or read book Microbial Versatility in Varied Environments written by Raghvendra Pratap Singh and published by Springer Nature. This book was released on 2020-04-09 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book compiles the latest studies on microorganisms thriving in extreme conditions. Microbes have been found in extremely high and low temperatures, highly acidic to saline conditions, from deserts to the Dead sea, from hot-springs to underwater hydrothermal vents- the diversity is incredible. The various chapters highlight the microbial life and describe the mechanisms of tolerance to these harsh conditions, and show how an understanding of these phenomena can help us exploit the microbes in biotechnology. The theme of the book is highly significant since life in these environments can give vital clues about the origin and evolution of life on earth, as a lot of these conditions simulate the environment present billions of years ago. Additionally, the study of adaptation and survival of organisms in such environments can be important for finding life on other planets. This book shall be useful for students, researchers and course instructors interested in evolution, microbial adaptations and ecology in varied environments.
Download or read book Compatible Solutes Engineering for Crop Plants Facing Climate Change written by Shabir Hussain Wani and published by Springer Nature. This book was released on 2021-10-30 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plants, being sessile and autotrophic in nature, must cope with challenging environmental aberrations and therefore have evolved various responsive or defensive mechanisms including stress sensing mechanisms, antioxidant system, signaling pathways, secondary metabolites biosynthesis, and other defensive pathways among which accumulation of osmolytes or osmo-protectants is an important phenomenon. Osmolytes with organic chemical nature termed as compatible solutes are highly soluble compounds with no net charge at physiological pH and nontoxic at higher concentrations to plant cells. Compatible solutes in plants involve compounds like proline, glycine betaine, polyamines, trehalose, raffinose family oligosaccharides, fructans, gamma aminobutyric acid (GABA), and sugar alcohols playing structural, physiological, biochemical, and signaling roles during normal plant growth and development. The current and sustaining problems of climate change and increasing world population has challenged global food security. To feed more than 9 billion, the estimated population by 2050, the yield of major crops needs to be increased 1.1–1.3% per year, which is mainly restricted by the yield ceiling. A major factor limiting the crop yield is the changing global environmental conditions which includes drought, salinity and extreme temperatures and are responsible for a reduction of crop yield in almost all the crop plants. This condition may worsen with a decrease in agricultural land or the loss of potential crop yields by 70%. Therefore, it is a challenging task for agricultural scientists to develop tolerant/resistant varieties against abiotic stresses. The development of stress tolerant plant varieties through conventional breeding is very slow due to complex multigene traits. Engineering compatible solutes biosynthesis by deciphering the mechanism behind the abiotic tolerance or accumulation in plants cell is a potential emerging strategy to mitigate adverse effects of abiotic stresses and increase global crop production. However, detailed information on compatible solutes, including their sensing/signaling, biosynthesis, regulatory components, underlying biochemical mechanisms, crosstalk with other signaling pathways, and transgenic development have not been compiled into a single resource. Our book intends to fill this unmet need, with insight from recent advances in compatible solutes research on agriculturally important crop plants.
Download or read book Polyextremophiles written by Joseph Seckbach and published by Springer Science & Business Media. This book was released on 2013-05-13 with total page 626 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many Microorganisms and some macro-organisms can live under extreme conditions. For example, high and low temperature, acidic and alkaline conditions, high salt areas, high pressure, toxic compounds, high level of ionizing radiation, anoxia and absence of light, etc. Many organisms inhabit environments characterized by more than one form of stress (Polyextremophiles). Among them are those who live in hypersaline and alkaline, hot and acidic, cold/hot and high hydrostatic pressure, etc. Polyextremophiles found in desert regions have to copy with intense UV irradiation and desiccation, high as well as low temperatures, and low availability of water and nutrients. This book provides novel results of application to polyextremophiles research ranging from nanotechnology to synthetic biology to the origin of life and beyond.
Download or read book Saline Soil based Agriculture by Halotolerant Microorganisms written by Manoj Kumar and published by Springer. This book was released on 2019-08-01 with total page 253 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses the role of salt in current agricultural approaches, including the low salt tolerance of agricultural crops and trees, impact of saline soils, and salt-resistant plants. Halophytes are extremely salt tolerant plants, which are able to grow and survive under salt at concentrations as high as 5 g/l by maintaining negative water potential. The salt-tolerant microbes inhabiting the rhizospheres of halophytes may contribute to their salt tolerance, and the rhizospheres of halophytic plants provide an ideal opportunity for isolating various groups of salt-tolerant microbes that could enhance the growth of different crops under salinity stress. The book offers an overview of salt-tolerant microbes' ability to increase plant tolerance to salt to facilitate plant growth, the potential of the halophytes’ rhizospheres as a reservoir of beneficial salt-tolerant microbes, their future application as bio-inoculants in agriculture and a valuable resource for an alternative way of improving crop tolerance to salinity and promoting saline soil-based agriculture. This special collection of reviews highlights some of the recent advances in applied aspects of plant (halophytes)-microbe interactions and their contribution towards eco-friendly approaches saline soil-based agriculture.
Download or read book Bacterial Stress Responses written by Gisela Storz and published by American Society for Microbiology Press. This book was released on 2010-11-16 with total page 1167 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gain new insight on utilizing bacterial stress responses to better combat bacterial infection with antibiotics and improve biotechnology. • Reviews the vast number of new findings that have greatly advanced the understanding of bacterial stress responses in the past 10 years. • Explores general regulatory principles, including the latest findings from genomics studies, including new research findings on both specific and general stress responses. • Details how stress responses affect the interactions between bacteria and host cells and covers bacterial stress responses in different niches and communities, with an emphasis on extreme environments.
Download or read book Salinity Responses and Tolerance in Plants Volume 2 written by Vinay Kumar and published by Springer. This book was released on 2018-06-22 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt: Soil salinity is a key abiotic-stress and poses serious threats to crop yields and quality of produce. Owing to the underlying complexity, conventional breeding programs have met with limited success. Even genetic engineering approaches, via transferring/overexpressing a single ‘direct action gene’ per event did not yield optimal results. Nevertheless, the biotechnological advents in last decade coupled with the availability of genomic sequences of major crops and model plants have opened new vistas for understanding salinity-responses and improving salinity tolerance in important glycophytic crops. Our goal is to summarize these findings for those who wish to understand and target the molecular mechanisms for producing salt-tolerant and high-yielding crops. Through this 2-volume book series, we critically assess the potential venues for imparting salt stress tolerance to major crops in the post-genomic era. Accordingly, perspectives on improving crop salinity tolerance by targeting the sensory, ion-transport and signaling mechanisms were presented in Volume 1. Volume 2 now focuses on the potency of post-genomic era tools that include RNAi, genomic intervention, genome editing and systems biology approaches for producing salt tolerant crops.
Download or read book Rhizobium Biology and Biotechnology written by Alexander P. Hansen and published by Springer. This book was released on 2017-09-25 with total page 339 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides in-depth reviews of the role of Rhizobium in agriculture and its biotechnological applications. Individual chapters explore topics such as: the occurrence and distribution of Rhizobium; phenotypic and molecular characteristics of Rhizobium; impact of Rhizobium on other microbial communities in the rhizosphere; N2-fixation ability of Rhizobium; Rhizobium and biotic stress; Rhizobium-mediated restoration of an ecosystem; in silico analysis of the rhizobia pool; further biotechnological perspectives of Rhizobium.
Download or read book Physiological and Biotechnological Aspects of Extremophiles written by Richa Salwan and published by Academic Press. This book was released on 2020-06-04 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: Physiological and Biotechnological Aspects of Extremophiles highlights the current and topical areas of research in this rapidly growing field. Expert authors from around the world provide the latest insights into the mechanisms of these fascinating organisms use to survive.The vast majority of extremophiles are microbes which include archaea, bacteria and some eukaryotes. These microbes live under chemical and physical extremes that are usually lethal to cellular molecules, yet they manage to survive and even thrive. Extremophiles have important practical uses. They are a valuable source of industrially important enzymes and recent research has revealed novel mechanisms and biomolecular structures with a broad range of potential applications in biotechnology, biomining, and bioremediation.Aimed at research scientists, students, microbiologists, and biotechnologists, this book is an essential reading for scientists working with extremophiles and a recommended reference text for anyone interested in the microbiology, bioprospecting, biomining, biofuels, and extremozymes of these organisms. Shows the implications of the physiological adaptations of microbes from extreme habitats that are largely contributed by their biomolecules from basic to applied research Provides in-depth knowledge of genomic plasticity and proteome of different extremophiles Gives detailed and comprehensive insight about use of genetic engineering as well as genome editing for industrial applications
Download or read book Microorganisms in Saline Environments Strategies and Functions written by Bhoopander Giri and published by Springer. This book was released on 2019-07-25 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book gathers the latest findings on the microbial ecology of saline habitats, plant-microbe interactions under saline conditions, and saline soil reclamation for agricultural use. The content is divided into four main parts: Part I outlines the definition of salinity, its genesis and impacts, and microbial diversity in saline habitats. Part II deals with impact of salinity on microbial and plant life/health. Part III highlights plant – microbe interactions in saline environments, and Part IV describes strategies for mitigation and reclamation of saline soils. The salinization of arable land is steadily increasing in many parts of the world. An excessive concentration of soluble salts (salinity) in soils or irrigation water adversely affects plant growth and survival. This problem is exacerbated in arid and semiarid areas due to their low precipitation and high evaporation rates. In turn, poor management practices and policies for using river water for the irrigation of agriculture crops often lead to the secondary salinization of soils. Considering the growing demands of a constantly expanding population, understanding the microbial ecology and interactions under saline conditions and their implications for sustainable agriculture is of utmost importance. Providing both an essential review of the status quo and a future outlook, this book represents a valuable asset for researchers, environmentalists and students working in microbiology and agriculture. .
Download or read book Plant Microbiome Stress Response written by Dilfuza Egamberdieva and published by Springer. This book was released on 2018-02-06 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents state-of-the-art research on the many facets of the plant microbiome, including diversity, ecology, physiology and genomics, as well as molecular mechanisms of plant-microbe interactions. Topics considered include the importance of microbial secondary metabolites in stimulating plant growth, induced systemic resistance, tolerance to abiotic stress, and biological control of plant pathogens. The respective contributions show how microbes help plants to cope with abiotic stresses, and represent significant progress toward understanding the complex regulatory networks critical to host-microbe interaction and plant adaptation in extreme environments. New insights into the mechanisms of microbial actions in inducing plant stress tolerance open new doors for improving the efficacy of microbial strategies, and could produce new ways of economically increasing crop yields without harming the environment. As such, this book offers an essential resource for students and researchers with an interest in plant-microbe interaction, as well as several possibilities for employing the plant microbiome in the enhancement of crop productivity under future climate change scenarios.
Download or read book Symbiotic Nitrogen Fixation written by P. Graham and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past three decades there has been a large amount of research on biological nitrogen fixation, in part stimulated by increasing world prices of nitrogen-containing fertilizers and environmental concerns. In the last several years, research on plant--microbe interactions, and symbiotic and asymbiotic nitrogen fixation has become truly interdisciplinary in nature, stimulated to some degree by the use of modern genetic techniques. These methodologies have allowed us to make detailed analyses of plant and bacterial genes involved in symbiotic processes and to follow the growth and persistence of the root-nodule bacteria and free-living nitrogen-fixing bacteria in soils. Through the efforts of a large number of researchers we now have a better understanding of the ecology of rhizobia, environmental parameters affecting the infection and nodulation process, the nature of specificity, the biochemistry of host plants and microsymbionts, and chemical signalling between symbiotic partners. This volume gives a summary of current research efforts and knowledge in the field of biological nitrogen fixation. Since the research field is diverse in nature, this book presents a collection of papers in the major research area of physiology and metabolism, genetics, evolution, taxonomy, ecology, and international programs.
Download or read book Soil Microbiomes for Sustainable Agriculture written by Ajar Nath Yadav and published by . This book was released on 2021 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book encompasses current knowledge of soil microbiomes and their potential biotechnological application for plant growth, crop yield, and soil health under the natural as well as harsh environmental conditions for sustainable agriculture. The microbes are ubiquitous in nature. The soil is a natural hotspot of the soil microbiome. The soil microbiome plays a critical role in the maintenance of global nutrient balance and ecosystem functioning. The soil microbiomes are associated with plant ecosystems through the intense network of plant-microbe interactions. The microbes present in bulk soil move toward the rhizospheric region due to the release of different nutrients by plant systems. The rhizospheric microbes may survive or proliferate in rhizospheric zone depending on the extent of influences of the chemicals secreted into the soil by roots. The root exudates contain the principal nutrients factors (amino acids, glucose, fructose, and sucrose). The microbes present in rhizospheric region have capabilities to fix atmospheric nitrogen, produce different phytohormones, and solubilize phosphorus, potassium, and zinc. The plant systems take these nutrients for their growth and developments. These soil and plant associated microbes also play an important role in protection of plants from different plant pathogenic organisms by producing different secondary metabolites such as ammonia, hydrogen cyanide, siderophores, and hydrolytic enzymes. The soil microbiomes with plant growth-promoting (PGP) attributes have emerged as an important and promising tool for sustainable agriculture. The soil microbiomes promote the plant growth and enhance the crop yield and soil fertility via directly or indirectly different plant growth-promoting mechanism. The soil microbes help the plant for adaptation in extreme habitats by mitigating the abiotic stress of high/low temperatures, hypersalinity, drought, and acidic/alkaline soil. These PGP microbes are used as biofertilizers/bioinoculants to replace the harmful chemical fertilizers for sustainable agriculture and environments. The aim of the book "Soil Microbiomes for Sustainable Agriculture" is to provide the recent advances in mechanisms of plant growth promotion and applications of soil microbiomes for mitigation of different abiotic stresses in plants. The book is useful to scientists, researchers, and students related to microbiology, biotechnology, agriculture, molecular biology, environmental biology, and related subjects.
Download or read book Microbes for Legume Improvement written by Almas Zaidi and published by Springer. This book was released on 2017-10-09 with total page 412 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents in-depth insights into strategies involving plant growth-promoting rhizobacteria (PGPR), including symbiotic/asymbiotic nitrogen fixers and associative/endophyte bacteria, phosphate-solubilizing microbes, as well as arbuscular mycorrhizal fungi and their active biomolecules in legume production. It also examines the latest research findings on the taxonomic status of rhizobia and signal molecules affecting rhizobia-legume symbiosis to improve readers’ understanding of the cultivation of legumes in conventional and derelict soil. The agronomically important microflora broadly discussed have offered solutions to some of the problems associated with expensive fertilizers used in many production systems. This second edition provides an overview of metal toxicity to legumes and presents strategies for the abatement of metal toxicity to legumes. Aimed at professionals, practitioners, researchers and graduate students in microbiology, crop sciences, soil microbiology, biotechnology and environmental microbiology, the book focuses on the basic concepts and practical aspects of useful soil microbiota in legume production.
Download or read book Advances in Soil Microbiology Recent Trends and Future Prospects written by Tapan Kumar Adhya and published by Springer. This book was released on 2018-03-15 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a comprehensive collection of articles illustrating the importance of microbial community structure and function for ecosystem sustainability and environmental reclamation. It addresses a diverse range of topics, including microbial diversity, physiology, genomics, ecosystem function, interaction, metabolism, and the fruitful use of microbial communities for crop productivity and environmental remediation. In addition, the book explores issues ranging from general concepts on the diversity of microorganisms in soil, and ecosystem function to the evolution and taxonomy of soil microbiota, with future prospects. It covers cutting-edge methods in soil microbial ecological studies, rhizosphere microflora, the role of organic matter in plant productivity, biological nitrogen fixation and its genetics, microbial transformation of plant nutrients in soil, plant-growth-promoting rhizobacteria, and organic matter transformation. The book also discusses the application of microbes in biodegradation of xenobiotic contaminants. It covers bio-fertilizers and their role in sustainable agriculture and soil health, biological control of insect pests and plant pathogens, and the latest tools of omics in soil microbiology, i.e. genomics, proteomics, transcriptomics and metabolomics, which offer pioneering approaches to the exploration of microbial structure and function.
