Download or read book A Laboratory Manual for the Training Course Recombinant DNA Techniques written by and published by . This book was released on 1989 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Recombinant DNA Laboratory Manual written by Judith W. Zyskind and published by Elsevier. This book was released on 2014-05-12 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recombinant DNA Laboratory Manual is a laboratory manual on the fundamentals of recombinant DNA techniques such as gel electrophoresis, in vivo mutagenesis, restriction mapping, and DNA sequencing. Procedures that are useful for studying either prokaryotes or eukaryotes are discussed, and experiments are included to teach the fundamentals of recombinant DNA technology. Hands-on computer sessions are also included to teach students how to enter and manipulate sequence information. Comprised of nine chapters, this book begins with an introduction to bacterial growth parameters, how to measure bacterial cell growth, and how to plot cell growth data. The discussion then turns to the isolation and analysis of chromosomal DNA in bacteria and Drosophila; plasmid DNA isolation and agarose gel analysis; and introduction of DNA into cells. Subsequent chapters deal with Tn5 mutagenesis of pBR329; DNA cloning in M13; DNA sequencing; and DNA gel blotting, probe preparation, hybridization, and hybrid detection. The book concludes with an analysis of lambda phage manipulations. This manual is intended for advanced undergraduate or beginning graduate students and should also be helpful to established investigators who are changing their research focus.
Download or read book Manipulation and Expression of Recombinant DNA written by Sue Carson and published by Elsevier. This book was released on 2005-12-15 with total page 172 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein—students can actually visualize positive clones following IPTG induction. *Cover basic concepts and techniques used in molecular biology research labs*Student-tested labs proven successful in a real classroom laboratories*Exercises simulate a cloning project that would be performed in a real research lab*"Project" approach to experiments gives students an overview of the entire process*Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Download or read book Molecular Biology Techniques written by Heather B. Miller and published by Academic Press. This book was released on 2011-10-18 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Download or read book Recombinant DNA Laboratory Manual written by Judith W. Zyskind and published by Morgan Kaufmann. This book was released on 1999 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Development of Simplified Recombinant DNA Technology Lab Manual for TBT 3010 biotechnology Course written by Marina Mokhtar and published by . This book was released on 2006 with total page 19 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Laboratory DNA Science written by Mark V. Bloom and published by Benjamin-Cummings Publishing Company. This book was released on 1996 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: This one-of-a-kind manual offers twenty-three foolproof labs designed to make molecular biology accessible and interesting to beginning biology students. Covering the basic techniques of gene manipulation and analysis, these "tried and true" experiments were tested and re-tested by the experienced author team to ensure absolute accuracy and ease of use.
Download or read book Basic Techniques in Molecular Biology written by Stefan Surzycki and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.
Download or read book DNA Science written by David A. Micklos and published by Cold Spring Harbor Laboratory Press. This book was released on 1990 with total page 504 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory text combines the theory, practice, and applications of recombinant DNA technology into one articulated package. Unlike super texts that can only be sampled by even the most ambitious instructor or student, DNA Science is designed to be read from cover to cover. The eight text chapters are written in a semi-journalistic style and adopt a historical perspective to explain where DNA science has come from and where it is going. Combining the unique perspectives of both a research biologist and a science writer, the topical treatment integrates up-to-the-minute examples drawn directly from the research literature. Extensively tested by thousands of high school and college teachers and students in 25 states and Canada, the ten laboratory experiments cover the basic techniques of gene isolation and analysis. The experiments engender systematic repetition to build student confidence and mastery of techniques. Extensive prelab notes at the beginning of each experiment explain how to schedule and prepare, and flowcharts and icons make the protocols easy to follow. The laboratory course is completely supported by quality-assured Carolina Biological Supply Company products -- from bulk reagents, to reusable reagent systems, to single-use kits -- satisfying a range of teaching applications. Truly a first course in recombinant DNA technology, the laboratory sequence presupposes no prior experience on the part of the instructor or student. Structured to follow directly from an introduction to principles of biology, the experiments are equally appropriate for the advanced high school student and the beginning college student. The book can be used as the first course in a molecularbiology sequence, be integrated as a genetics/DNA structure component of a general biology course, or be used as a unit within a microbiology or genetics course. The text is suitable for introducing recombinant DNA in science and society courses.
Download or read book DNA Science written by David A. Micklos and published by . This book was released on 1988 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Biotechnology written by Bernard R. Glick and published by . This book was released on 1998 with total page 724 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition explains the principles of recombinant DNA technology as well as other important techniques such as DNA sequencing, the polymerase chain reaction, and the production of monclonal antibodies.
Download or read book Recombinant DNA and Biotechnology written by Helen Kreuzer and published by . This book was released on 1996 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the last edition was published, more European legislation has been incorporated into the law of the United Kingdom, and the third edition contains a full account of the 1992 regulations implementing European directives. The Treaty of Amst"
Download or read book Molecular Biology written by Susan J. Karcher and published by Academic Press. This book was released on 1995-11-28 with total page 303 pages. Available in PDF, EPUB and Kindle. Book excerpt: This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented. Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning. - Includes a glossary of over 200 terms important for understanding molecular biology - Uses an inexpensive source of eukaryotic cells - great for schools on a budget - Includes Methods Locator that provides instant access to the latest methods - Contain clearly written, easy-to-follow, student-tested instructions: - Sterile techniques - Phage titration - Gel electrophoresis of DNA - Restriction enzyme digestion - Plasmid isolation - Transformation of E. Coli - Recombinant DNA cloning - Nick translation labeling - Nonradioactive primer labelling - Nonradioactive DNA detection - Southern blotting - Colony hybridization - Purification of plant DNA - RNA purification - Northern blotting - Purification of poly A+ RNA - Polymerase chain reaction (PCR)
Download or read book Recombinant DNA Technical Bulletin written by and published by . This book was released on 1982 with total page 478 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Cloning written by Joseph Sambrook and published by . This book was released on 1989 with total page 752 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Experiments in Molecular Biology written by Zachary F. Burton and published by Academic Press. This book was released on 1997-02-20 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: Experiments in Molecular Biology provides a thorough introduction to recombinant DNA methods used in molecular biology and nucleic acid biochemistry. This unique laboratory manual is particularly appropriate for courses in molecular cloning, molecular genetics techniques, molecular biology techniques, recombinant DNA techniques, bacterial genetics techniques, and genetic engineering. Included is an especially helpful section to aid new instructors in avoiding potential pitfalls of specific experiments. Key Features * Contains student-tested, easy-to-follow protocols * Presents background information that reinforces principles behind the methods presented * Includes questions at the end of laboratory exercises * Provides both detailed descriptions of experimental procedures and a theoretical support section * Sequentially links experiments to provide a "project" approach to studying molecular biochemistry * Includes student-tested, easy-to-follow protocols * Background information reinforces principles behind the methods presented * Includes questions at the end of laboratory exercises * Advises new instructors on potential pitfalls of specific experiments * Provides both detailed descriptions of experimental procedures and a theoretical support section * Sequentially links experiments to provide a "project" approach to studying
Download or read book Methods in Plant Molecular Biology written by Mary A. Schuler and published by Academic Press. This book was released on 2012-12-02 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Plant Molecular Biology is a lab manual that introduces students to a diversity of molecular techniques needed for experiments with plant cells. Those included have been perfected and are now presented for the first time in a usable and teachable form. Because the manual integrates protein, RNA, and DNA techniques, it will serve students, teachers, and researchers in plant physiology, biophysics, and animal molecular biology who have no previous experience handling recombinant DNA or purified proteins. It can also be used by the established molecular biologist who wishes to utilize the powerful techniques of recombinant DNA to explore the mysteries of the plant kingdom. - Eight basic experiments which can be used collectively or individually cover - Recombinant Cloning and Screening in E. coli; DNA Sequencing - Plant RNA Isolation and in Vitro Translations - Plant DNA Isolations and Genomic DNA Southern AnalysisChloroplast Isolation and Protein SynthesisPlant Tissue Culture and Agrobacterium Transformations - Experiments that have been student tested for three years - Blueprints for setting up gel rigs - Comprehensive course schedule outlining individual procedures to be finished in each lab segment - Course can be tailored to suit the needs of the individual instructor
