Download or read book A Genetic Analysis to Elucidate the Function of the Plasmodium Falciparum Parasitophorous Vacuole Protein PfPV1 written by Trang T. T. Chu and published by . This book was released on 2009 with total page 244 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Genetic Analysis to Elucidate the Function of the Plasmodium Falciparum Parasitophorous Vacuole Protein PfPV1 written by and published by . This book was released on 2009 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Malaria is one of the most lethal infectious diseases worldwide. Understanding the biology of the causative agent Plasmodium will lead to better control of the disease. The biogenesis and maintenance of the parasitophorous vacuole (PV) within the infected erythrocyte is an essential factor for parasite survival. The PV has been postulated to be involved in various pivotal functions, however little is known about the PV contents and their respective functions. Our group had previously provided the first PV's proteome research and have continuously exposed more members of this important compartment. The protein PfPV1 was a newly discovered PV localisation protein, encoded by the PF11_0302 gene. In order to address the function of this protein a gene knock-out strategy was applied. A search for the interacting proteins of PfPV1 was also carried out using the GST pull-down assay. The first attempt to knock out the encoding gene was the double-crossover strategy in the presence of a negative selection. The knocked-out parasite was unable to obtain. However, the integration into the PfPV1 locus did occur, evidenced by the presence of both endogenous and knock-out band in the specific southern blot hybridisation. The PfPV1 gene was therefore assumed to be essential for in vitro growth, thus the targeting into the endogenous locus was accompanied by a duplication event for maintaining expression of the gene. The assumption was further validated by the second knock-out strategy, using the complementing experiment. The result had been expected to be able to disrupt the endogenous gene locus through the knock-out vector while concomitantly expressing a copy of PfPV1 under the control of a foreign promoter from an episomal plasmid, which should not recombine with the endogenous gene locus. However, the gene was still resistant to be disrupted. Various clones were isolated from the double transfected parasites. One of the clones has lost the episomal copy of the PfPV1 gene and s.
Download or read book Drug Discovery and Drug Development written by Madhu Dikshit and published by Springer. This book was released on 2022-02-11 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the years, India has attained a prominent global position in the manufacture of Generic Drugs. This success can be attributed to its synthetic organic chemistry and chemical engineering strengths, nurtured by the timely policies of the Government of India. However, breakthrough successes in New Drug Discovery have remained elusive, despite the brilliant and sustained efforts of many Indian researchers and Pharma establishments. The Indian National Science Academy thought it appropriate to document India’s New Drug Discovery Research (NDDR) journey to date. Gathering contributions from prominent researchers in the Indian Pharma Industry and Academia, this book highlights their efforts, achievements, and the status quo of Indian NDDR.
Download or read book The Problem with Survey Research written by George Beam and published by Transaction Publishers. This book was released on 2012-08-14 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Problem with Survey Research makes a case against survey research as a primary source of reliable information. George Beam argues that all survey research instruments, all types of asking—including polls, face-to-face interviews, and focus groups—produce unreliable and potentially inaccurate results. Because those who rely on survey research only see answers to questions, it is impossible for them, or anyone else, to evaluate the results. They cannot know if the answers correspond to respondents’ actual behaviors (objective phenomena) or to their true beliefs and opinions (subjective phenomena). Reliable information can only be acquired by observation, experimentation, multiple sources of data, formal model building and testing, document analysis, and comparison. In fifteen chapters divided into six parts—Ubiquity of Survey Research, The Problem, Asking Instruments, Asking Settings, Askers, and Proper Methods and Research Designs—The Problem with Survey Research demonstrates how asking instruments, settings in which asking and answering take place, and survey researchers themselves skew results and thereby make answers unreliable. The last two chapters and appendices examine observation, other methods of data collection and research designs that may produce accurate or correct information, and shows how reliance on survey research can be overcome, and must be.
Download or read book Business Research Methods written by S Sreejesh and published by Springer Science & Business Media. This book was released on 2013-07-31 with total page 291 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since research is best learned by doing, this book emphasizes a hands-on, do-it yourself approach. The readers have many opportunities to see how business researches affect and support management decision. The book used a case study approach for all the chapters with interactive videos. The book gave emphasis to quantitative data analysis using a software program, IBM SPSS 20.0. The data analysis chapters illustrate in detail each step in running the software programs. The software programs files are provided for all data sets: outputs, demonstration movies, and screen captures are on the Website. This book provides students most extensive help available to learn quantitative data analysis using SPSS. Thus, the authors prepared this textbook and all the additional materials to help the students to understand the functional principles of business research and how to apply them in real-life situations.
Download or read book Management Research written by Susan Rose and published by Routledge. This book was released on 2014-07-25 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt: For many post-graduate students undertaking a research project for the first time is a daunting prospect. Gaining the knowledge and skills needed to do research typically has to be done alongside carrying out the project itself. Students often have to conduct their research independently, perhaps with limited tutor contact. What is needed in such situations is a resource that supports the new researcher on every step of the research journey, from defining the project to communicating its findings. Management Research: Applying the Principles provides just such a resource. Structured around the key stages of a research project, it is designed to provide answers to the questions faced by new researchers but without neglecting the underlying principles of good research. Each chapter includes ‘next steps’ activities to help readers apply the content to their own live research project. The companion website provides extensive resources, including video tutorials, to support the development of practical research skills. The text reflects the richness and variety of current business and management research both in its presentation of methods and techniques and its choice of examples drawn from different subject disciplines, industries and organizations. Management Research: Applying the Principles combines diversity of coverage with a singularity of purpose: to help students complete their research project to a rigorous standard.
Download or read book Organizational Pathology written by Yitzhak Samuel and published by Transaction Publishers. This book was released on 2011-12-01 with total page 189 pages. Available in PDF, EPUB and Kindle. Book excerpt: Organizational Pathology draws an extended metaphor that the life cycle of an organization is akin to the biological life cycle. Like all living things, organizations will encounter problems that lead to decline and eventual failure. This work discusses the basic problems and life threatening diseases responsible for organizations' failure and death, including organizational politics, organizational corruption, and organizational crime. The book also contains a critical look at crises and fixations; failure and survival; and processes of disbandment and closure of dying organizations. The consideration of these issues follows a diagnostic model of failure. Yitzhak Samuel argues that if the problems that lead to failure can be predicted or diagnosed early, their severity can be assessed and possible remedies can be implemented to avoid escalating crises. At the very least, an understanding of why and how decline happens can be gained from this analysis. This book offers facts about the causes and consequences of organizational downfall and clues about diagnoses of certain symptoms of abnormal behavior, and how to identify early signs of decline or failure. In order to illustrate these abstract arguments and concepts, Samuel uses various real-life examples of events that have occurred in cross-country contexts. In this way, Organizational Pathology: Life and Death of Organizations should serve a variety of readers. Although primarily intended for students and scholars in the social and behavioral sciences who are familiar with the study and the practice of organizations, this book's informal style makes it easily accessible to a wide range of readers. Just as Samuel's previous book on organizational politics led to new lines of research and theory, this book will encourage similar studies in organizational pathology and institutional malaise.
Download or read book Entrepreneurial Marketing written by Robert D. Hisrich and published by Edward Elgar Publishing. This book was released on 2018 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: One key for success of an entrepreneur is to obtain sales (revenue) and profits as quickly as possible upon launching the venture. Entrepreneurial Marketing focuses on the essential elements of success in order to achieve these needed sales and revenues and to grow the company. The authors build a comprehensive, state-of-the-art picture of entrepreneurial marketing issues, providing major theoretical and empirical evidence that offers a clear, concise view of entrepreneurial marketing. Through an international approach that combines both theoretical and empirical knowledge of entrepreneurship and marketing, this book informs and enhances the entrepreneurs' creativity, their ability to bring innovations to the market, and their willingness to face risk that changes the world. Key components addressed include: identifying and selecting the market; determining the consumer needs cost-effectively; executing the basic elements of the marketing mix (product, price, distribution, and promotion); and competing successfully in the domestic and global markets through implementing a sound marketing plan. Numerous illustrative examples throughout the book bring the content to life. The mix of theoretical content, examples, empirical analyses, and case studies make this book an excellent resource for students, professors, researchers, practitioners, and policymakers all over the world.
Download or read book Molecular Chaperones in the Cell written by Peter A. Lund and published by Oxford University Press, USA. This book was released on 2001 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this text, leading experts synthesise our body of knowledge , and the reader gains not only a fuller understanding of the roles of chaperones in the context of cellular processes, but also an insight into the nature of these proteins.
