Download or read book A Functional Analysis of the RNA Polymerase II Large Subunit Carboxy terminal Domain written by Rob Chapman and published by . This book was released on 2002 with total page 122 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Functional Analysis of the RNA Polymerase II C terminal Domain Kinase Ctk1 in the Yeast Saccharomyces Cerevisiae written by Susanne Röther and published by . This book was released on 2007 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Functional Significance of the Carboxyl terminal Domain of RNA Polymerase IIa o written by Paul James Laybourn and published by . This book was released on 1989 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book RNA Polymerase II Carboxy terminal Domain Phosphatase written by Michael S. Kobor and published by . This book was released on 2001 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The form of RNA polymerase II (RNAPII) that binds preferentially to promoters is not extensively phosphorylated on the carboxy-terminal heptapeptide repeat domain (CTD) of its largest subunit. The CTD becomes phosphorylated during or shortly after initiation and elongating RNAPII generally has a phosphorylated CTD. Prior to or following transcriptional termination, dephosphorylation of the CTD presumably must occur to regenerate the hypophosphorylated form of RNAPII that is capable of reinitiating transcription. This thesis examines the function of the CTD phosphatase Fcp1p in the yeast 'Saccharomyces cerevisiae'. In chapter 2, it is shown that Fcp1 is an unusual eukaryotic protein phosphatase that is required for dephosphorylation of the CTD 'in vivo ' and for transcription by RNAPII 'in vivo'. These results suggest that Fcp1p is the founding member of a new class of protein phosphatases and acts as a general transcription factor 'in vivo'. In chapter 3, affinity chromatography is used to study the binding of Fcp1p to TFIIB and the RAP74 subunit of TFIIF. Fcp1p binds in a similar way to both of these factors. RAP74 and TFIIB have a short region of homology and amino acid changes in this region affect the binding to Fcp1p. The genes encoding RAP74 and Fcp1p interact 'in vivo'. Fcp1p can activate transcription when artificially tethered to a promoter and this effect is largely dependent on binding to RAP74. In chapter 4, it is shown that yeast strains with mutations in ' fcp1' grow much worse when the gene encoding the major CTD kinase Kin28p is also mutated. In contrast, inactivation of another CTD kinase encoded by the 'SRB10' gene suppresses the temperature-sensitivity and the sensitivity to certain cell cycle checkpoint inducing drugs of ' fcp1' mutant strains. These results therefore suggest that Fcp1p and Srb10p have opposing roles 'in vivo'. In chapter 5, analysis of the phosphorylation state of the CTD reveals that reduced Fcp1p activity results in a increased amount of the largest subunit of RNAPII but this subunit is not incorporated into functional enzyme and is largely degraded at a higher temperature.
Download or read book A Mutational Analysis of the Carboxyl terminal Domain of the RNA Polymerase II Largest Subunit written by Marilyn A. L. West and published by . This book was released on 1992 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book RNA Polymerase and Associated Factors Part C written by Sankar Adhya and published by Elsevier. This book was released on 2003-12-18 with total page 833 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA polymerase is molecule important to gene transcription. Along with associated factors, RNA polymerase is part of the process in which RNA is transcribed to produce a protein.* Construction and purification of RNA polymerases* DNA microarrays and bacterial gene expression* Functional analysis of transcription factors
Download or read book Functional Analysis of the Ninth Subunit of Yeast RNA Polymerase II RPB9 written by and published by . This book was released on 1999 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods in Yeast Genetics written by David C. Amberg and published by CSHL Press. This book was released on 2005 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Methods in Yeast Genetics" is a course that has been offered annually at Cold Spring Harbor for the last 30 years. This provides a set of teaching experiments along with the protocols and recipes for the standard techniques and reagents used in the study of yeast biology.
Download or read book Mutant Analysis of the RNA Polymerase II Carboxy terminal Domain written by Lauren McCarl and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The carboxy-terminal domain (CTD) of Rpb1, the largest subunit of RNA polymerase II (RNAPII), is a tail-like extension that enables RNAPII to interact with a myriad of factors essential for transcription and co-transcriptional RNA processing. It is characterized by tandem heptad repeats, the consensus sequence of which is YSPTSPS. Different combinations of phosphorylated residues have been found to correlate with different stages of the transcription cycle. To enhance our understanding of the functionally enigmatic nonconsensus heptads, two distinct generations of CTD mutant transgenic fly lines were generated by the Gilmour lab. The first generation expressed both endogenous RNAPII and a CTD mutant version of the polymerase. The second generation, designed to determine rescue ability, co-expressed short hairpin RNA against endogenous Rpb1 mRNA (shRpb1) and a shRpb1-resistant version of CTD mutant RNAPII. In addition to helping construct several plasmids to create these CTD mutants, I also characterized previously generated mutants by beta-galactosidase ([beta]-gal) staining assay and quantitative real-time PCR (qRT-PCR). The [delta]Hep(exp) mutant, characterized by the deletion of a highly conserved, eight-repeat region containing the only two conserved heptads of the Drosophila CTD, was of particular interest because it is lethal in adult flies. My [beta]-gal assays and qRT-PCR analysis showed the [delta]Hep(exp) mutation had largely no effect on hsp70 reporter gene expression or endogenous hsp70 mRNA synthesis, respectively. This suggests the defect associated with [delta]Hep(exp) may involve a gene (or genes) other than hsp70.
Download or read book Functional Analysis of the Ninth Subunit of Yeast RNA Polymerase II RPB9 written by Sally Anne Hemming and published by . This book was released on 1998 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Immunological and Functional Analysis of RNA Polymerase II from Caenorhabditis Elegans written by Joseph P. Prenger and published by . This book was released on 1987 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The C terminal Domain of RNA Polymerase II written by Anton Yuryev and published by . This book was released on 1995 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Biology of RNA written by Masayori Inouye and published by . This book was released on 1987 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Biology of RNA: New Perspectives provides an overview of the developments in RNA research as well as the approaches, strategies, and methodologies used. Most of the contributing authors in the present volume participated in the Fifth Stony Brook Symposium entitled ""New Perspectives on the Molecular Biology of RNA"" in May 1986. The text is organized into six parts. Part I contains papers dealing with RNA as an enzyme. Part II presents studies on RNA splicing. Part III examines RNA viruses while Part IV focuses on the role of RNA in DNA replication. Part V is devoted to the structure ...
Download or read book The Operon written by Jeffrey H. Miller and published by . This book was released on 1980 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Phosphorylation of the Carboxyl terminal Domain of the RNA Polymerase II Largest Subunit written by Jie Zhang and published by . This book was released on 1991 with total page 98 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Specific Interaction Between the Carboxy Terminal Domain of the Largest Subunit of RNA Polymerase II and the Tata Box Binding Protein written by Christo B. Houbaviy and published by . This book was released on 1993 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Computation Finite and Infinite Machines written by Marvin Lee Minsky and published by Prentice Hall. This book was released on 1967 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt:
