Download or read book A Cell Culture Model for Understanding Estrogen Receptor Regulation in Normal and Malignant Cells Addendum written by and published by . This book was released on 2001 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: One of the major obstacles to study the contribution of estrogen receptor (ER) to the initiation and progression of breast cancer is the lack of an appropriate culture model that duplicates the estrogenic effects seen in the normal gland in vivo. We postulate that the correct expression and function of ER depends on an appropriate microenvironment that preserves cell-cell and cell-matrix interactions. Here we determined that the extracellular matrix (ECM) modulates the expression and function of the ER. The stimulation of the ER- mediated response by the presence of cell-matrix interactions involves the a6 integrin subunit. Using quantitative RT-PCR and western blots we also demonstrated that both normal and tumorigenic mouse mammary cell lines in culture increased ERa expression levels in response to ECM and that those levels are higher in the tumorigenic cell line. Moreover, the presence of estradiol decreased the expression of its own receptor in both cell types, two-fold in normal cells and fifteen-fold in tumorigenic cells. Therefore, we believe that the regulatory effect of ECM is exerted, at least in part, by modulating both the ER-mediated response and/or ERa levels itself. In addition, we have developed an in vitr0 culture system for mouse mammary cells isolated from the gland, which partly prevents the loss of ERa expression normally seen in standard culture conditions. This improved technique relies on culturing the cells in 3D cultures inside collagen I gels or on top of Matrigel instead of 2D monolayers, and the addition of lactogenic hormones. These results suggest that the approach that we have chosen to analyze the regulation of ER function by a three-dimensional matrix is promising. The regulation of ER by ECM could be a crucial step in the progression of breast cancer from an ER-positive to an ER- negative tumor, which leads to a hormone-independent and more aggressive cancer phenotype. In addition, manipulating ER expression in mammary cells b7.

Download or read book A Cell Culture Model for Understanding Estrogen Receptor Regulation in Normal and Malignant Cells written by and published by . This book was released on 1998 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: To characterize a spontaneous epithelial-to-mesenchymal conversion (EMT) in the non- transformed "functional normal" mammary epithelial cell line SCp2. Determine if the mesenchymal conversion in SCp2 cells is associated with tumorigenesis. Determine if alterations in growth factor expression and regulation are accompanied by changes in ECM and metalloproteinases expression. Determine if the presence of 3D structure is critical to stopping spontaneous and induced conversion.

Download or read book Hormonal Contraception and Post menopausal Hormonal Therapy written by IARC Working Group on the Evaluation of Carcinogenic Risks to Humans and published by World Health Organization. This book was released on 1999 with total page 692 pages. Available in PDF, EPUB and Kindle. Book excerpt: Evaluates evidence for an increased risk of cancer in women using combined oral contraceptives, progestogen-only hormonal contraceptives, post-menopausal estrogen therapy, and post-menopausal estrogen-progestogen therapy. Although the carcinogenicity of these preparations has been extensively investigated, the book stresses the many complex methodological issues that must be considered when interpreting findings and weighing results. Evidence of an association between use of these preparations and positive effects on health, including a reduced risk of some cancers, is also critically assessed. The first and most extensive monograph evaluates evidence of an association between the use of combined oral contraceptives and cancer at nine sites. Concerning breast cancer, the evaluation concludes that, even if the association is causal, the excess risk for breast cancer associated with patterns of use that are typical today is very small. Studies of predominantly high-dose preparations found an increased risk of hepatocellular carcinoma in the absence of hepatitis viruses. Citing these findings, the evaluation concludes that there is sufficient evidence in humans for the carcinogenicity of combined oral contraceptives. The evaluation also found sufficient evidence for the carcinogenicity of some, but not all, combined preparations in animals. Combined oral contraceptives were classified as carcinogenic to humans. The evaluation also cites conclusive evidence that these agents have a protective effect against cancers of the ovary and endometrium. Progestogen-only contraceptives are evaluated in the second monograph, which considers the association with cancer at six sites. The evaluation found no evidence of an increased risk for breast cancer. Although the evaluation found sufficient evidence in animals for the carcinogenicity of medroxyprogesterone acetate, evidence for the carcinogenicity of progestogen-only contraceptives in humans was judged inadequate. Progestogen-only contraceptives were classified as possibly carcinogenic to humans. The third monograph, on post-menopausal estrogen therapy, considers evidence of an association with cancer at eight sites. Findings from a large number of epidemiological studies indicate a small increase in the risk of breast cancer in women who have used these preparations for five years or more. Studies consistently show an association between use of post-menopausal estrogen therapy and an increased risk for endometrial cancer. Data on the association with other cancers were either inconclusive or suggested no effect on risk. The evaluation concludes that post-menopausal estrogen therapy is carcinogenic to humans. The final monograph evaluates the association between the use of post-menopausal estrogen-progestogen therapy and cancer at four sites. The evaluation of limited data on breast cancer found an increased relative risk observed with long-term use. Data were judged insufficient to assess the effects of past use and of different progestogen compounds, doses, and treatment schedules. For endometrial cancer, the evaluation found an increase in risk relative to non-users when the progestogen was added to the cycle for 10 days or fewer. Post-menopausal estrogen-progestogen therapy was classified as possibly carcinogenic to humans. Concerning post-menopausal therapy in general, the book notes that evidence of carcinogenic risks must be placed in perspective of potential benefits. The prevention of osteoporotic fractures is cited as the best-established benefit. Evidence also suggests that estrogen prevents heart disease and may prevent memory loss and dementia.

Download or read book ICD 10 CM Official Guidelines for Coding and Reporting FY 2021 October 1 2020 September 30 2021 written by Department Of Health And Human Services and published by Lulu.com. This book was released on 2020-09-06 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt: These guidelines have been approved by the four organizations that make up the Cooperating Parties for the ICD-10-CM: the American Hospital Association (AHA), the American Health Information Management Association (AHIMA), CMS, and NCHS. These guidelines are a set of rules that have been developed to accompany and complement the official conventions and instructions provided within the ICD-10-CM itself. The instructions and conventions of the classification take precedence over guidelines. These guidelines are based on the coding and sequencing instructions in the Tabular List and Alphabetic Index of ICD-10-CM, but provide additional instruction. Adherence to these guidelines when assigning ICD-10-CM diagnosis codes is required under the Health Insurance Portability and Accountability Act (HIPAA). The diagnosis codes (Tabular List and Alphabetic Index) have been adopted under HIPAA for all healthcare settings. A joint effort between the healthcare provider and the coder is essential to achieve complete and accurate documentation, code assignment, and reporting of diagnoses and procedures. These guidelines have been developed to assist both the healthcare provider and the coder in identifying those diagnoses that are to be reported. The importance of consistent, complete documentation in the medical record cannot be overemphasized. Without such documentation accurate coding cannot be achieved. The entire record should be reviewed to determine the specific reason for the encounter and the conditions treated.

Download or read book Alternative Toxicological Methods written by Harry Salem and published by CRC Press. This book was released on 2003-03-26 with total page 616 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bringing together the recent and relevant contributions of over 125 scientists from industry, government, and academia in North America and Western Europe, Alternative Toxicological Methods explores the development and validation of replacement, reduction, and refinement alternatives (the 3Rs) to animal testing. Internationally recognized scientist

Download or read book European Guidelines for Quality Assurance in Breast Cancer Screening and Diagnosis written by European Commission. Directorate-General for Health and Consumer Protection and published by . This book was released on 2006 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: Breast cancer is the most frequent cause of cancer-related deaths in women in Europe, and demographic trends indicate a continuing increase in this substantial public health problem. Systematic early detection through screening, effective diagnostic pathways and optimal treatment have the ability to substantially lower current breast cancer mortality rates and reduce the burden of this disease in the population. This is the fourth edition of these guidelines which contains information on recommended standards and procedures for breast cancer screening and diagnostic services, including chapters on multi-disciplinary aspects of quality assurance, data collection and monitoring, effective communication of information, requirements of a specialist unit, and a certification protocol.

Download or read book Toxicological Profile for Chlordane written by and published by . This book was released on 1994 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular and Cellular Effects of Nutrition on Disease Processes written by Grant N. Pierce and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains the proceedings of the 2nd World Conference of the International Society for Molecular Nutrition & Therapy. This conference was held on August 2-4, 1997, in Winnipeg, Canada. The goal of the conference was to advance our knowledge concerning the molecular events which link nutrition to various disease processes in the body. This volume represents an important compilation of unique articles addressing the molecular and cellular basis for the nutritional and therapeutic treatment of five general disease processes.

Download or read book Toxicological Profile for Nitrophenols written by and published by . This book was released on 1992 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Nuclear Receptor FactsBook written by Vincent Laudet and published by Gulf Professional Publishing. This book was released on 2002 with total page 486 pages. Available in PDF, EPUB and Kindle. Book excerpt: The FactsBook Series has established itself as the best source of easily accessible and accurate facts about protein groups. They use an easy-to-follow format and are researched and compiled by experts in the field. This Factsbook is devoted to nuclear receptors. The first section presents an introduction and describes the mode of action of the receptors in general. The second section of the book contains detailed entries covering each type of receptor. Entries provide information on: Nomenclature and structure, Isolation, DNA binding properties, Ligands, Expression, Target genes, Knockouts, Disease association, Gene structure, promoter and isoforms, Chromosomal location, Amino acid sequences, Key references

Download or read book Toxicological Profile for Aldrin dieldrin written by and published by . This book was released on 2002 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetics of Colorectal Cancer written by John D. Potter and published by Springer. This book was released on 2008-12-08 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetic susceptibility refers to how variations in a person’s genes increase or decrease his or her susceptibility to environmental factors, such as chemicals, radiation and lifestyle (diet and smoking). This volume will explore the latest findings in the area of genetic susceptibility to gastrointestinal cancers, focusing on molecular epidemiology, DNA repair, and gene-environment interactions to identify factors that affect the incidence of GI cancers. Topics will include germline susceptibility, including Mendelian patterns of inheritance and gene-environment interactions that lead to cancer etiology.

Download or read book Toxicological Profile for Styrene written by and published by . This book was released on 1992 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Toxicological Profile for Isophorone written by and published by . This book was released on 1989 with total page 120 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Modification of Radiation Response written by Carsten Nieder and published by Springer Science & Business Media. This book was released on 2003 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes and summarizes the radiation responses of both normal and neoplastic tissues with a focus on rational strategies for the modification of these responses. Emerging data from molecular oncology and radiobiology are reviewed in depth. The book covers not only general principles of radiation-induced reactions but also a large number of preclinical and clinical data that will guide the reader through this complex and dynamic field and will provide valuable information for the development of further research projects.

Download or read book Toxicological Profile for Beryllium written by and published by . This book was released on 2002 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Toxicological Profile for Pentachlorophenol written by and published by . This book was released on 2001 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt: