Download or read book A Basic Study to Assess the Potential Usefulness of Resonance Raman Spectroscopy as a Means of Rapidly Detecting and Identifying Bacteria and Other Microorganisms written by W. H. Nelson and published by . This book was released on 1985 with total page 15 pages. Available in PDF, EPUB and Kindle. Book excerpt: This past year we have explored the potential uses of Resonance Raman and Fluorescence Lifetime Spectroscopies in the rapid characterization of bacteria. Previously we have shown that chromobacteria can be distinguished on the basis of distinctive resonance Raman spectra. Spectra are excited by low power argon ion laser radiation at 488 nm and are due to the presence of carotenoid pigments. While carotenoid pigments in bacteria generally are not useful for identification purposes, the study of chromobacteria has allowed us to assess the potential sensitivity of resonance Raman spectroscopy in this application. We were able to laser illuminate and count two types of bacteria under a microscope. Because it was possible to see the laser excited bacteria while the resonance Raman spectra were being obtained, it was possible to estimate closely the number of bacteria producing a given spectrum. Such high sensitivity suggests but does not prove that spectra are 'surface enhanced'. It is clear that the resonance Raman technique can be highly sensitive and our experiments suggest that remote detection and detection from mixtures is possible in principle.
Download or read book Scientific and Technical Aerospace Reports written by and published by . This book was released on 1985 with total page 1152 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Research in Progress written by and published by . This book was released on 1983 with total page 644 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Research in Progress written by United States. Army Research Office and published by . This book was released on 1984 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1977- consist of two parts: Chemistry, biological sciences, engineering sciences, metallurgy and materials science (issued in the spring); and Physics, electronics, mathematics, geosciences (issued in the fall).
Download or read book The Rapid Detection of Bacteria and Other Microorganisms A Basic Study in the Application of Resonance Raman and Time Resolved Fluorescence Spectroscopies written by Wilfred H. Nelson and published by . This book was released on 1988 with total page 22 pages. Available in PDF, EPUB and Kindle. Book excerpt: Resonance Raman Spectra have been obtained for a variety of chromobacteria using low power 488 nm excitation. Spectra are simple, of high quality, and useful for identification purposes at the species level. Raman microprobe studies show conclusively that spectra can be obtained from single cells in pure cultures or in mixed cultures without need for separation. Extensions of the study have been made to representative colorless gram-negative and gram-positive bacteria. Spora other than bacteria have been studied as well. Pollen, mold spores, bacterial spores, algae and viruses all give spectra but only viruses and bacterial spores appear to give intense UV Resonance Raman spectra. The primary fluorescence of bacteria has been studied in detail to determined its potential in rapid detection. We have determined fluorescence for S. epidermidis, P. fluorescens, E. cloacae, E. coli and B. subtilis. Fluorescence contributions have been assigned in part to tryptophan, pteridines, related flavins and pyridine coenzymes.
Download or read book Government Reports Announcements Index written by and published by . This book was released on 1988-10 with total page 504 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Aerospace Medicine and Biology written by and published by . This book was released on 1986 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: A selection of annotated references to unclassified reports and journal articles that were introduced into the NASA scientific and technical information system and announced in Scientific and technical aerospace reports (STAR) and International aerospace abstracts (IAA).
Download or read book Application of Raman Spectroscopy for Studying Bacteria of the Family Enterobacteriaceae written by Amir Nakar and published by . This book was released on 2023* with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thesis investigated using Raman spectroscopy for identifying Enterobacteriaceae and their antibiotic resistances. The study aimed to develop a rapid, accurate diagnostic tool, as current methods are costly and inefficient, leading to increased antibiotic resistance due to inappropriate antibiotic use. The research involved collecting a dataset of Enterobacteriaceae, encompassing 8 genera. Measurements were taken using Single-Cell Raman Microspectroscopy (SC-RMS) and Fiber Probe-Based Raman Spectroscopy (FP-RS). Machine learning models were trained to classify the bacteria based on spectral data. SC-RMS showed limited success (47% accuracy), while FP-RS achieved 100% accuracy on the training dataset and 94% on a validation dataset, demonstrating its potential in differentiating Enterobacteriaceae. Subsequently, Raman spectra of 24 clinical isolates were analyzed using UV-Resonance Raman Spectroscopy (UVRRS). The goal was to discriminate between E. coli, K. pneumoniae, and K. oxytoca. While SC-RMS had limited effectiveness, UVRRS achieved high accuracy (92% at the genus level and 90% at the species level), marking the first successful differentiation of K. oxytoca using Raman spectroscopy. The study also explored differentiating antibiotic-resistant and sensitive Enterobacteriaceae. Both SC-RMS and UVRRS correctly classified 70-75% of the isolates, with UVRRS demonstrating a 90% sensitivity for resistant strains. This differentiation may be related to a higher DNA/Protein ratio in resistant bacteria. Overall, the findings enhance the application of Raman spectroscopy in microbiology, particularly for Enterobacteriaceae. The research supports its use in identifying pathogens, determining their genus and species, and assessing antibiotic resistance. The study contributes significantly to the development of Raman-based microbial diagnostics, promising to improve infectious disease diagnostics, patient care, and the fight against antimicrobial resistance.
Download or read book Raman Spectroscopy and Applications written by Khan Maaz and published by BoD – Books on Demand. This book was released on 2017-02-15 with total page 366 pages. Available in PDF, EPUB and Kindle. Book excerpt: Raman spectroscopy has a number of applications in various fields including material science, physics, chemistry, biology, geology, and medicine. This book illustrates necessary insight and guidance in the field of Raman spectroscopy with detailed figures and explanations. This presents deep understanding of new techniques from basic introduction to the advance level for scientists and engineers. The chapters cover all major aspects of Raman spectroscopy and its application in material characterization with special emphasis on both the theoretical and experimental aspects. This book is aimed to provide solid foundation of Raman spectroscopy to the students, scientists, and engineers working in various fields as mentioned above.
Download or read book Halophilic Microorganisms and their Environments written by Aharon Oren and published by Springer Science & Business Media. This book was released on 2006-04-06 with total page 571 pages. Available in PDF, EPUB and Kindle. Book excerpt: "This water" he told me, "runs out to the eastern region, and flows into the Arabah; and when it comes into the sea, into the sea of foul waters [i. e. , the Dead Sea], the water will become wholesome. Every living creature that swarms will be able to live wherever this stream goes; the fish will be very abundant once these waters have reached there. It will be wholesome, and everything will live wherever this stream goes. Fishermen shall stand beside it all the way from En-gedi to En-eglaim; it shall be a place for drying nets; and the fish will be of various kinds [and] most plentiful, like the fish of the Great Sea. " Ezekiel’s prophecy (Ezekiel 47: 8-10) for revival and purification of the Dead Sea waters This new book on "Halophilic Microorganisms and their Environments" is the fifth volume in the COLE series (Cellular Origin and Life in Extreme Habitats (see: http://www. wkap. nl/prod/s/COLE). In the previous books we covered aspects of enigmatic microorganisms, microbial diversity, astrobiology, and symbiosis, so this book on halophilic microbes adds a fitting link to the rest of series' books. Since ancient times hypersaline habitats have been considered extreme environments, and some were thought not to sustain life at all. Yet, every organism requires salt for its existence. Salty places have been compared to an environment of extinction (e. g. , the Dead Sea).
Download or read book Stimulated Raman Scattering Microscopy written by Ji-Xin Cheng and published by Elsevier. This book was released on 2021-12-04 with total page 612 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stimulated Raman Scattering Microscopy: Techniques and Applications describes innovations in instrumentation, data science, chemical probe development, and various applications enabled by a state-of-the-art stimulated Raman scattering (SRS) microscope. Beginning by introducing the history of SRS, this book is composed of seven parts in depth including instrumentation strategies that have pushed the physical limits of SRS microscopy, vibrational probes (which increased the SRS imaging functionality), data science methods, and recent efforts in miniaturization. This rapidly growing field needs a comprehensive resource that brings together the current knowledge on the topic, and this book does just that. Researchers who need to know the requirements for all aspects of the instrumentation as well as the requirements of different imaging applications (such as different types of biological tissue) will benefit enormously from the examples of successful demonstrations of SRS imaging in the book. Led by Editor-in-Chief Ji-Xin Cheng, a pioneer in coherent Raman scattering microscopy, the editorial team has brought together various experts on each aspect of SRS imaging from around the world to provide an authoritative guide to this increasingly important imaging technique. This book is a comprehensive reference for researchers, faculty, postdoctoral researchers, and engineers. Includes every aspect from theoretic reviews of SRS spectroscopy to innovations in instrumentation and current applications of SRS microscopy Provides copious visual elements that illustrate key information, such as SRS images of various biological samples and instrument diagrams and schematics Edited by leading experts of SRS microscopy, with each chapter written by experts in their given topics
Download or read book Microbial Bioprocesses written by Pratyoosh Shukla and published by Elsevier. This book was released on 2023-04-20 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbial processes are involved in food, chemical, pharmaceutical, cosmetics, energy, and new-material industries. Over the past 2 decades, new or more efficient industrial processes involving microorganisms have been launched, yielding purer, less expensive products or substances not available using classical chemical methods. Microbial Bioprocesses aims to give an overview of established and successful processes and discusses the trends and perspectives in industrial microbiology which, along with tremendous progress in genetic and metabolic engineering in recent years, are once again becoming an area of innovation and emerging technologies. Microbial Bioprocesses covers the unique areas like microbial volatiles (MVOCs), microbial bioinoculant development, bacterial nanocelluloses production, and processes for remediation by fungi and actinobacteria. Provides an innovative description of emerging bioprocesses for biotechnological gains Provides a broad cross section of microbial bioprocess that covers several sustainability applications Gives general insights about how microorganisms can be utilized in production of various chemicals
Download or read book Raman Spectroscopy for Chemical Analysis written by Richard L. McCreery and published by John Wiley & Sons. This book was released on 2005-02-25 with total page 451 pages. Available in PDF, EPUB and Kindle. Book excerpt: Minimaler Aufwand bei der Probenvorbereitung, hoher Informationsgehalt des Spektrums und die Möglichkeit, mit festen Proben zu arbeiten, machen die Raman-Spektroskopie zunehmend attraktiv. Wie man diese Methode mit modernster Ausrüstung effizient anwendet, zeigt Ihnen das vorliegende Buch. Im Mittelpunkt stehen neue Entwicklungen wie CCDs, Diodenlaser und Fourier-Transform-Techniken. Behandelt werden auch quantitative Analysen, die in der bisher vorhandenen Literatur häufig zu kurz kamen. (08/00)
Download or read book A Resonance Raman Method for Rapid Detection Identification and Quantitation of Bacteria in Sewage and Natural Waters written by Wilfred H. Nelson and published by . This book was released on 1979 with total page 36 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Applications of Raman Spectroscopy to Biology written by M. Ghomi and published by IOS Press. This book was released on 2012-02-23 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: Raman spectroscopy has been known and used as a technique for 80 years, originally for the study of inorganic substances. Recent advances in underlying technology, such as lasers, detectors, filters and components, have transformed the technique into a very effective modern tool for studying complex biological problems. Professor Mahmoud Ghomi (of the University of Paris XIII) has edited this book on the applications of Raman spectroscopy to biology, covering in a readily accessible way the area from basic studies to the diagnosis of disease. The early chapters provide background information on basic principles underlying the main Raman methods covered in the book, with information on Surface-Enhanced Raman Scattering (SERS) and Surface-Enhanced Fluorescence (SEF), as well as giving accounts of applications to biomolecular and cellular investigations. Among the topics covered are studies of drugs and their complexes with biomolecules on nanoparticles, application of SERS to blood analysis, studies of single cells and of applications to human cancer diagnostics.This will be a useful book for experimental scientists in academic, governmental, industrial and clinical environments and for those entering the field of biomolecular spectroscopy.
Download or read book Novel Food Preservation and Microbial Assessment Techniques written by Ioannis S. Boziaris and published by CRC Press. This book was released on 2014-04-14 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: Demand for minimally processed foods has resulted in the development of innovative, non-thermal food preservation methods, such as high-pressure sonication, ozone, and UV treatment. This book presents a summary of these novel food processing techniques. It also covers new methods used to monitor microbial activity, including spectroscopic methods (
Download or read book Applications of UV Resonance Raman and Micro Raman Spectroscopy for the Rapid Characterization of Bacteria and the Study of Polymers written by Suneet Chadha and published by . This book was released on 1993 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt:
